Sci Rep:武漢大學丁毅教授研究組發表蓮線粒體基因組研究成果

更新時間:2022-04-02 15:43:36 所屬欄目:基因組 作者:金盈玫

摘要:近日,國際學術權威刊物自然出版集團旗下子刊《ScientificReports》雜志在線發表了武漢大學生命科學學院雜交水稻國家重點實驗室丁毅教授團隊題為「ThemitochondrialgenomemapofNelumbonuciferarevealsancientevoluti

近日,國際學術權威刊物自然出版集團旗下子刊《Scientific Reports》雜志在線發表了武漢大學生命科學學院雜交水稻國家重點實驗室丁毅教授團隊題為 「The mitochondrial genome map of Nelumbo nucifera reveals ancient evolutionary features」

的研究論文。該論文首次完成了蓮線粒體基因組的全基因組測序和線粒體基因組物理圖的組裝,并揭示了蓮作為基部雙子葉植物的古老進化特征。該論文共同第一作者為博士生桂松濤和博士吳智華,通訊作者為丁毅教授。

作為孓遺植物的代表之一,對蓮的基因組學的研究對于探索高等被子植物進化具有十分重要的意義。在該項研究中,作者利用第三代基因組單分子測序技術(SMRT)對蓮的線粒體基因組進行了測序、組裝和注釋,得到了蓮線粒體基因組序列和完整基因組圖譜,并揭示了蓮線粒體基因組具有進化上的保守性特征,即保留著古老的基因簇和基因數目、高頻率的RNA編輯以及低頻率的葉綠體片段的插入等現象。這是丁毅教授課題組對蓮基因組學研究的系列工作之一,作為國家自然科學基金資助的蓮基因組研究計劃的一部分工作,蓮線粒體基因組圖譜的完成,為全面理解蓮基因組特征以及探索被子植物進化途徑提供了新的線索,也豐富了被子植物基因組進化的數據資料。

研究組今年成果:2013年參與完成了蓮( Nelumbo nucifera Gaertn.)核基因組測定(The Plant Journal 2013,76:557-567)、2014年完成蓮葉綠體基因組測定(BMC plant biology,2014, 14:289)、2016年5月完成了蓮著絲粒蛋白和著絲粒DNA序列的特征研究(The Plant

Journal)。

原文連結:

原文摘要:

Nelumbo nucifera is an evolutionary relic from the Late Cretaceous period. Sequencing the N. nucifera mitochondrial genome is important for elucidating the evolutionary characteristics of basal

eudicots. Here, the N. nucifera mitochondrial genome was sequenced using single molecule real-time sequencing technology (SMRT), and the mitochondrial genome map was constructed after de novo

assembly and annotation. The results showed that the 524,797-bp N. nuciferamitochondrial genome has a total of 63 genes, including 40 protein-coding genes, three rRNA genes and 20 tRNA genes. Fifteen

collinear gene clusters were conserved across different plant species. Approximately 700 RNA editing sites in the protein-coding genes were identified. Positively seleced genes were identified with

selection pressure analysis. Nineteen chloroplast-derived fragments were identified, and seven tRNAs were derived from the chloroplast. These results suggest that the N. nucifera mitochondrial genome

retains evolutionarily conserved characteristics, including ancient gene content and gene clusters, high levels of RNA editing, and low levels of chloroplast-derived fragment insertions. As the first

publicly available basal eudicot mitochondrial genome, the N. nucifera mitochondrial genome facilitates further analysis of the characteristics of basal eudicots and provides clues of the

evolutionary trajectory from basal angiosperms to advanced eudicots.

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